Sufficient biological replication is essential for differential expression analysis of RNA-seq

I just took part in a twitter discussion about the trade-offs between sequencing depth and number of independent biological replicate (per treatment group) for differential gene expression analysis. While there are applications of RNA-seq where  sequencing deeply (more than say 50 million reads for a given sample) can be important for discovery. However, most researchers …

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Implementing Discovery

This post is the first (of two) about my suggestions for how to implement “open discovery” for answering scientific questions, but in a way that does not completely alienate current professional scientists. In particular because of the current system of how “credit” for answering questions translates to prestige which directly translates to tangible materialistic considerations …

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pictures in my head: What is that on the wing of the fly? What does it tell us about adaptation?

Over the past week or so, there has been an absolutely amazing image that has made the rounds on the internet of a fly (Goniurellia tridens)with markings on its wings reminiscent to many viewers of ants. Goniurellia tridens is a 3-in-1 insect [photo: Peter Roosenschoon] pic.twitter.com/i8ThAOkrvN — Ziya Tong (@ziyatong) November 4, 2013 As described …

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