Ex Laboratorium

The E. coli long-term evolution experiment, or LTEE for short, is approaching its 30th birthday, which will be on February 24th, 2018. In honor of all the people who have worked on this project, I thought it would be neat to commission a special, but shareable, piece of art. Given the history of science and …

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If

Every day, we propagate the E. coli populations in the long-term evolution experiment (LTEE) by transferring 0.1 ml of the previous day’s culture into 9.9 ml of fresh medium. This 100-fold dilution and regrowth back to stationary phase—when the bacteria have exhausted the resources—allow log2 100 = 6.64 generations (doublings) per day. We round that …

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Some Wrinkles in Time

Today is another milestone for the E. coli long-term evolution experiment—the LTEE, for short. I did the 10,000th daily transfer today at about noon. [Yours truly, doing the 10,000th LTEE transfers. Technician Neerja Hajela is keeping a close eye on me, and with good reason. Photo by Thomas LaBar.] Some of you will remember we …

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Another Birthday Haiku

As I said in my last post, I just celebrated my 60th birthday with lots of friends and family. Several folks produced new artistic works, including two lovely haikus that celebrate the E. coli long-term evolution experiment. Here’s one from Mike Wiser, who did his doctoral research on the long-term lines. A highlight of his …

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63,000 Strong

Ever wonder about those big numbers posted in a window in that tall building on the east side of Farm Lane, across from the entrance to the MSU Dairy Store? Right now, the digits read 63000. That’s the number of generations in an experiment that’s been running in my lab for over a quarter century. …

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Tiny Things that Live in Little Bottles

As I mentioned in my previous post, it can be a fun challenge to explain your scientific research to people who aren’t scientists. A week or so ago I came across a website that challenges you to explain something complicated using only the thousand most commonly used words. So here’s my effort about our long-term evolution experiment with E. …

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How good is MEGAHIT?

A few weeks back, Nick Loman (via Manoj Samanta) brought MEGAHIT to our attention on Twitter. MEGAHIT promised “an ultra-fast single-node solution for large and complex metagenome assembly” and they provided a preprint and some open source software. This is a topic near and dear to my heart (see Pell et al., 2012 and Howe …

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